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SUMOylated Golgin45 associates with PML-NB to transcriptionally regulate lipid metabolism genes during heat shock stress

Commun Biol. 2024-05; 
Shuaiyang Jing, Jingkai Gao, Neeraj Tiwari, Yulei Du, Lianhui Zhu, Bopil Gim, Yi Qian, Xihua Yue, Intaek Lee
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Proteins, Expression, Isolation and Analysis … The supernatants were incubated with indicated antibodies and protein G agarose beads (L00209, GenScript) for 4 h at 4 C with end-over-end agitation. The beads were washed two … Get A Quote

摘要

Golgin tethers are known to mediate vesicular transport in the secretory pathway, whereas it is relatively unknown whether they may mediate cellular stress response within the cell. Here, we describe a cellular stress response during heat shock stress via SUMOylation of a Golgin tether, Golgin45. We found that Golgin45 is a SUMOylated Golgin via SUMO1 under steady state condition. Upon heat shock stress, the Golgin enters the nucleus by interacting with Importin-β2 and gets further modified by SUMO3. Importantly, SUMOylated Golgin45 appears to interact with PML and SUMO-deficient Golgin45 mutant functions as a dominant negative for PML-NB formation during heat shock stress, suppressing transcription of lipid m... More

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