| Products/Services Used | Details | Operation |
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| Proteins, Expression, Isolation and Analysis | … The supernatants were incubated with indicated antibodies and protein G agarose beads (L00209, GenScript) for 4 h at 4 C with end-over-end agitation. The beads were washed two … | Get A Quote |
Golgin tethers are known to mediate vesicular transport in the secretory pathway, whereas it is relatively unknown whether they may mediate cellular stress response within the cell. Here, we describe a cellular stress response during heat shock stress via SUMOylation of a Golgin tether, Golgin45. We found that Golgin45 is a SUMOylated Golgin via SUMO1 under steady state condition. Upon heat shock stress, the Golgin enters the nucleus by interacting with Importin-β2 and gets further modified by SUMO3. Importantly, SUMOylated Golgin45 appears to interact with PML and SUMO-deficient Golgin45 mutant functions as a dominant negative for PML-NB formation during heat shock stress, suppressing transcription of lipid m... More
