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A second open reading frame in human enterovirus determines viral replication in intestinal epithelial cells

Nat Commun.. 2019; 
Guo H1,2, Li Y2, Liu G2, Jiang Y3, Shen S2, Bi R1, Huang H3, Cheng T4, Wang C1, Wei W5,6.
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摘要

Human enteroviruses (HEVs) of the family Picornaviridae, which comprises non-enveloped RNA viruses, are ubiquitous worldwide. The majority of EV proteins are derived from viral polyproteins encoded by a single open reading frame (ORF). Here, we characterize a second ORF in HEVs that is crucial for viral intestinal infection. Disruption of ORF2p expression decreases the replication capacity of EV-A71 in human intestinal epithelial cells (IECs). Ectopic expression of ORF2p proteins derived from diverse enteric enteroviruses sensitizes intestinal cells to the replication of ORF2p-defective EV-A71 and respiratory enterovirus EV-D68. We show that the highly conserved WIGHPV domain of ORF2p is important for ORF2p-dep... More

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